Transfection:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
Clone Assay:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
Plasmid Preparation:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
Modification:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
Expressing:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
Sequencing:Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a β-actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3′-UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer's suggested protocol.
Article Title: Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse
Article Snippet: .. QMCs were transfected with plasmids encoding mouse PUM2 (pCMV-Sport6 mPUM2, Open Biosystems) or PUM2-Cherry fusion protein (chicken PUM2 fused with mCherry cloned in the NotI sites of pIRES vector previously modified to include a -actin promoter for expression in eukaryotic systems). pEGFP-C1 (Clontech) was used to clone the full-length AChE 3 -UTR after the GFP-coding sequence in the EcoRI site. .. Plasmids were transfected using Exgen-500 (Fermentas) following the manufacturer’s suggested protocol.
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